Reverse Transcription:Article Title: Targeted Inhibition of CCL22 by miR-130a-5p Can Enhance the Sensitivity of Cisplatin-Resistant Gastric Cancer Cells to Chemotherapy
Article Snippet: Firstly, the total RNA in tissues and cells was extracted with Trizol reagent (Baiaolaibo Technology Co., Ltd., Beijing, China, QN-2070-ZOG). .. Then, 5 μg of total RNA was taken, respectively, according to the instructions of kits (Beijing Huada Protein Research and Development Center Co., Ltd., China, BPI01030) for reverse transcription cDNA operation, and 1 μL of synthesized cDNA was taken after transcription for amplification. .. Amplification system was as follows: cDNA 1 μL, upstream and downstream primers 0.4 μL each, 2X TransScript ® Tip Green qPCR SuperMix 10 μL, Passive Reference Dye (50X) 0.4 μL, Nuclease-free Water made up to 20 μL.
Synthesized:Article Title: Targeted Inhibition of CCL22 by miR-130a-5p Can Enhance the Sensitivity of Cisplatin-Resistant Gastric Cancer Cells to Chemotherapy
Article Snippet: Firstly, the total RNA in tissues and cells was extracted with Trizol reagent (Baiaolaibo Technology Co., Ltd., Beijing, China, QN-2070-ZOG). .. Then, 5 μg of total RNA was taken, respectively, according to the instructions of kits (Beijing Huada Protein Research and Development Center Co., Ltd., China, BPI01030) for reverse transcription cDNA operation, and 1 μL of synthesized cDNA was taken after transcription for amplification. .. Amplification system was as follows: cDNA 1 μL, upstream and downstream primers 0.4 μL each, 2X TransScript ® Tip Green qPCR SuperMix 10 μL, Passive Reference Dye (50X) 0.4 μL, Nuclease-free Water made up to 20 μL.
Amplification:Article Title: Targeted Inhibition of CCL22 by miR-130a-5p Can Enhance the Sensitivity of Cisplatin-Resistant Gastric Cancer Cells to Chemotherapy
Article Snippet: Firstly, the total RNA in tissues and cells was extracted with Trizol reagent (Baiaolaibo Technology Co., Ltd., Beijing, China, QN-2070-ZOG). .. Then, 5 μg of total RNA was taken, respectively, according to the instructions of kits (Beijing Huada Protein Research and Development Center Co., Ltd., China, BPI01030) for reverse transcription cDNA operation, and 1 μL of synthesized cDNA was taken after transcription for amplification. .. Amplification system was as follows: cDNA 1 μL, upstream and downstream primers 0.4 μL each, 2X TransScript ® Tip Green qPCR SuperMix 10 μL, Passive Reference Dye (50X) 0.4 μL, Nuclease-free Water made up to 20 μL.
|
